The sybr green i dye chemistry uses the sybr green i dye to detect pcr products by binding to double stranded dna formed during pcr. We have observed that assays run in kicqstart readymix are optimal when using a higher primer concentration than in conventional pcr. Itaq universal sybr green supermix is a 2x concentrated ready to use reaction master mix optimized for dye based quantitative pcr qpcr on any real time pcr instrument.
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